ASC-patches, empty gelatin sponge or a standard dressing of silicone/polyurethane were sutured on a full thickness wound on the ischemic foot of Wistar rats. Error bars refer to SD.ĪSC-patches close ischemic wounds faster than acellular gelatin sponge and silicon/polyurethane dressings, with an increased vascularization of the granulation tissue. ( A) Heatmap of the top 25 genes with increased expression and the top 25 with decreased expression in the ASC-patch compared with ASC monolayer condition, ranked by Gene Set Enrichment Analysis (GSEA): The regulations considered to be significant were defined by the calculation of a fold change (> or 2 (up) and <2 (down) and p < 0.05, non-parametric Mann–Whitney t test). The transcriptome of ASC in gelatin generated from 3 independent donors were assessed by microarray and compared to ASC from the same 3 donors grown in monolayer. This formulation of ASC represents an optimal, convenient and clinically compliant option for the delivery of ASC on ischemic wounds.Īdipose stem cells clinical translation mesenchymal stem cells stromal cells.ĪSCs within the ASC-patch enhanced their regenerative transcriptome. In conclusion, we provide here preclinical evidence that a cross-linked porcine gelatin sponge is an optimal carrier to concentrate and increase the regenerative activity of ASC, notably angiogenic. Finally, ASC in a gelatin sponge prepared from outbred rats accelerated closure and re-vascularization of ischemic wounds in the footpads of rats. No tumor formation was observed in immunodeficient nude mice to which an ASC gelatin sponge was transplanted subcutaneously. ASC in gelatin on a chick chorioallantoic membrane were more connected to vessels than an empty sponge, confirming an increased angiogenesis in vivo. This secretome promoted fibroblast survival and epithelialization, and significantly increased the migration and tubular assembly of endothelial cells within fibronectin. Proteomics on conditioned media showed that gelatin also acted as a concentrator and reservoir of the regenerative ASC secretome. Wound healing genes were massively regulated, notably with the inflammatory and angiogenic factors. Transcriptomic data revealed that, compared to ASC in a monolayer, a cross-linked porcine gelatin sponge strongly influences the transcriptome of ASC. This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |